T5293
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 | Immunoblotting Whole cell extract of Chicken fibroblasts was separated on SDS-PAGE, and blotted with monoclonal Anti-ß Tubulin (Cat. No. T5293). The antibody was developed with Goat APA-Mouse Fab, Peroxidase conjugate (Cat. No. A9917) and AEC substrate. Lanes A: Antibody dilution 1:200 B: Negative control (only secondary antibody) |
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T5293
Sigma
Monoclonal Anti-β-Tubulin antibody produced in mouse
clone 2-28-33, ascites fluid
| MDL number: | MFCD00145892 |
Description
| Immunogen | sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes. |
| Specificity | The antibody recognizes the two major and one of the minor β‑tubulin isotypes of the free-living soil nematode C. elegans. It also binds to Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus sperm axonemes, to neuronal axons of the larva of an ascidian (Ciona intestinalis) and to tubulin of chicken and mammals, cultured human fibroblasts, bovine and rat brain tissue. In C.elegans preparations, the antibody does not recognize tubulin that has been heat denatured in the presence of SDS and β-mercaptoethanol, though it is bound to tubulin separated on isoelectric focusing gels. It reacts with heat-denatured and reduced mammalian preparations in immunoblotting. |
Properties
| antibody form | ascites fluid |
| clone | 2-28-33 (monoclonal) |
| contains | 15 mM sodium azide |
| species reactivity | rat, chicken, bovine, Caenorhabditis elegans, Strongylocentrotus purpuratus, Ciona intestinalis, human |
| application(s) | immunoblotting: suitable |
| immunocytochemistry: suitable | |
| indirect immunofluorescence: 1:1,000 using cultured human fibroblasts | |
| isotype | IgG1 |
| Gene Information | human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383) |
| rat ... Tubb2(29212), Tubb2c(296554) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Siddiqui, S.S., et al. J. Neurosci. 9, 2963, (1989) Abstract |
| Crowther, R.J., and Whittaker, J.R. J. Neurobiol. 23, 280, (1992) Abstract | |
| Ahmed, A., et al., Viral fusogenic membrane glycoproteins kill solid tumor cells by nonapoptotic mechanisms that promote cross presentation of tumor antigens by dendritic cells Cancer Res. 62, 6566-6578, (2002) Abstract | |
| Mole, D.R., et al., Characterization of different isoforms of the HIF prolyl hydroxylase PHD1 generated by alternative initiation Biochem. J. 397, 179-186, (2006) Abstract | |
| Calaghan, S., et al., Cardiac microtubules are more resistant to chemical depolymerisation in streptozotocin-induced diabetes in the rat Pflugers Arch. 444, 432-437, (2002) Abstract | |
| Qian Li-Wu, et al., Kaposi's Sarcoma-Associated Herpesvirus Disrupts Adherens Junctions and Increases Endothelial Permeability by Inducing Degradation of VE-Cadherin J. Virol. 82, 11902-11912, (2008) |
